Thermo Fisher Scientific 6X His Tag Monoclonal Antibody (33D10.D2.G8), Atto 550
6X His 태그 단백질 검출용 마우스 단클론 항체로 Atto 550 형광으로 결합됨. Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 적합. 비특이적 반응이 낮고 고감도 검출 가능. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
| Immunoprecipitation (IP) | >1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 33D10.D2.G8 |
| Immunogen | HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide. |
| Conjugate | Atto™ 550 |
| Excitation / Emission Max | 554 / 575 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Available Formats
- Unconjugated
- Atto 647N
- Biotin
- Cyanine3
- Cyanine5
- DyLight 405
- DyLight 488
- DyLight 549
- DyLight 649
- DyLight 680
- DyLight 800
- HRP
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
6X HIS Epitope Tag ATTO 550 conjugated antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. It has reacted with all His-tagged proteins tested and does not cross-react with endogenous bacterial proteins. The antibody recognizes His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of target proteins in transfected or transformed cells.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at the N- or C-terminal of recombinant proteins to facilitate purification via immobilized metal affinity chromatography (IMAC). The strong and selective affinity of histidine residues to Ni²⁺ enables effective purification using Ni²⁺-NTA adsorbent. His tag-specific antibodies are used to detect or co-immunoprecipitate tagged proteins when no specific antibody is available.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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