Thermo Fisher Scientific CD34 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
Suggested positive controls: angiosarcoma, lung, or tonsil tissue.

Target Information

CD34 is a highly glycosylated monomeric surface protein (111–115 kDa) expressed on various stem cell populations. It serves as a stem cell marker, with expression reversible in human hematopoietic stem cells.
CD34 may function as a surface receptor involved in receptor-mediated endocytosis and regulation of adhesion, differentiation, and proliferation of hematopoietic and progenitor cells.
It likely mediates attachment of stem cells to the bone marrow extracellular matrix or stromal cells and may act as a scaffold for glycans binding to lectins.
The intracellular domain can be phosphorylated by protein kinase C, suggesting a role in signal transduction.
Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.