Thermo Fisher Scientific Alexa Fluor 488 Recombinant Rabbit Monoclonal Antibody (15H9L93)
Alexa Fluor 488에 특이적인 recombinant rabbit monoclonal antibody로, 높은 특이성과 민감도를 제공합니다. ICC/IF, Flow Cytometry 등 다양한 응용에 사용 가능하며, lot 간 일관성과 우수한 재현성을 보장합니다. 연구용으로만 사용됩니다.
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Applications 및 Tested Dilution
| Application | Tested Dilution | References |
|---|---|---|
| Immunocytochemistry (ICC/IF) | Assay-Dependent | - |
| Flow Cytometry (Flow) | Assay-Dependent | - |
| Neutralization (Neu) | Assay-Dependent | - |
| Fluorescent Quenching (FQ) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Expression System | ExpiCHO |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 15H9L93 |
| Immunogen | Alexa Fluor® 488 dye conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090464 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select the best production candidates.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Broad immunoreactivity due to the large rabbit immune repertoire
Target Information
Anti-fluorescent dye antibodies recognize specific fluorophores and often quench their fluorescence. These antibodies, including those recognizing fluorescein, can serve as cell-impermeant probes to determine whether fluorescent dye-conjugated ligands, proteins, bacteria, or other biomolecules have been internalized by endocytic or pinocytic processes.
Alexa Fluor® 488 dye exhibits spectral properties and quantum yield nearly identical to FITC but provides brighter, more photostable conjugates, making it ideal for imaging and applications requiring high sensitivity and stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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