Thermo Fisher Scientific Alexa Fluor 488 Recombinant Rabbit Monoclonal Antibody (15H9L93)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Flow Cytometry (Flow)

Assay-Dependent

-

Neutralization (Neu)

Assay-Dependent

-

Fluorescent Quenching (FQ)

Assay-Dependent

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Rabbit / IgG

Expression System

ExpiCHO

Class

Recombinant Monoclonal

Type

Antibody

Clone

15H9L93

Immunogen

Alexa Fluor® 488 dye conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3090464

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes. The Alexa Fluor® 488 dye possesses near identical spectral properties and quantum yield to fluorescein isothiocyanate (FITC), but produces brighter, more photostable conjugates. These conjugates are used for imaging and other applications requiring increased sensitivity without being overly impacted by environmental factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.