Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), NovaFluor Blue 510, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: ≤ 0.4 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 661, 737
• Brilliant Violet 421, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• NovaFluor Blue 610-70S, Blue 660-120S, Red 685, Red 710, Yellow 570, Yellow 590, Yellow 660
• Functional Grade
• Custom conjugation available

Product Specific Information

The M5/114.15.2 monoclonal antibody reacts with mouse MHC Class II glycoproteins (I-A and I-E subregions: I-Aᵇ, I-Aᵈ, I-Aᵠ, I-Eᵈ, I-Eᵏ). It detects determinants on B cells, monocytes, macrophages, dendritic cells, and activated T cells from mice carrying H-2ᵇ, H-2ᵈ, H-2ᵠ, H-2ᵖ, H-2ʳ, H-2ᵘ haplotypes, but not H-2ˢ or H-2ᶠ.

• Inhibits I-A–restricted T cell responses in H-2ᵇ, H-2ᵈ, H-2ᵠ, H-2ᵘ haplotypes
• Each product includes 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤0.4 µg/test (100 µL assay volume, 10⁵–10⁸ cells/test)

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• Always use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) with NovaFluor conjugates.
  • Add 5 µL per sample or per 100 µL of cell sample.
  • Add buffer to antibody cocktails before mixing with cells.

NovaFluor conjugates utilize Phiton™ technology based on novel nucleic acid dye structures, engineered to minimize spillover and spread.
Excitation: 496 nm; Emission: 511 nm; Laser: 488 nm (Blue)

Target Information

MHC Class II molecules are transmembrane glycoproteins expressed on professional antigen-presenting cells such as macrophages, dendritic cells, and B cells. They bind endocytosed exogenous antigens and present them to T cells. The antigen-binding groove is open at both ends and accommodates peptides 15–24 amino acids long.
MHC/HLA antibodies are key tools in Immunopeptidomics, aiding in neoantigen identification via LC-MS/MS.

For Research Use Only. Not for diagnostic use or resale without authorization.

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