Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), NovaFluor Blue 660-120S, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: ≤ 0.4 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Red 685 / 700 / 755
• NovaFluor Yellow 660 / 700
• PE / PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 702
• Custom conjugation available

Product Specific Information

The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110–120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle, and epithelial cells. CD29 forms non-covalent complexes with alpha integrins (CD49a–f) to create VLA-1 through VLA-6 complexes and interacts with CD51. These heterodimers mediate cell adhesion, trafficking, proliferation, and differentiation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse bone marrow cells (≤ 0.4 µg/test, 100 µL final volume)

Recommended titration for optimal performance.
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01)

• Required for optimal staining with NovaFluor conjugates
• Add 5 µL per sample before combining with cells
• For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells)

NovaFluor Conjugate Technology
NovaFluor dyes utilize Phiton™ technology with engineered nucleic acid dye structures to minimize spillover and improve spectral separation.

• Excitation: 509 nm
• Emission: 665 nm
• Laser: 488 nm (Blue)

Target Information

ITGB1 (Integrin Subunit Beta 1 / CD29)
A 110 kDa cell surface glycoprotein expressed by various cell types including leukocytes. Forms heterodimers with multiple alpha chains (α1–α6) to define β1 (VLA) integrins. Plays key roles in:

• Cell adhesion and migration
• Immune cell trafficking
• Regulation of cytokine secretion
• Tumor cell drug resistance
• Inflammatory responses (e.g., IBD)

Two major isoforms exist: β1A (ubiquitous) and β1D (cardiac/skeletal muscle-specific).

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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