Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), NovaFluor Blue 660-120S

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: ≤ 0.4 µg/test

Species Reactivity

• Mouse
• Rat

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Red 685 / 700 / 755
• NovaFluor Yellow 660 / 700
• PE / PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 702
• Request custom conjugation

Product Specific Information

Description:
The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110–120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle, and epithelial cells. CD29 forms heterodimers with alpha integrins (CD49a–f, CD51), mediating adhesion, trafficking, proliferation, and differentiation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:
Suitable for flow cytometric analysis.

Applications Tested:
Validated for flow cytometry of mouse bone marrow cells at ≤ 0.4 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Antibody titration recommended for optimal performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.
  • Add 5 µL per sample before mixing with cells.
  • For single-color controls, add 5 µL per 100 µL of cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology—engineered fluorescent signatures optimized for minimal spillover and spread.

• Excitation: 509 nm
• Emission: 665 nm
• Laser: 488 nm (Blue)

Target Information

ITGB1 (Integrin Subunit Beta 1, CD29):
A 110 kDa cell surface glycoprotein expressed on various cell types, including leukocytes. Forms heterodimers with multiple alpha chains (α1–α6) to create VLA integrins, mediating adhesion to extracellular matrix proteins (collagen, laminin, fibronectin, vitronectin).

ITGB1 is involved in:

• Immune cell trafficking
• Tissue remodeling and inflammation
• Tumor cell drug resistance
• Cytokine secretion regulation via fibronectin and collagen interactions

Two major isoforms exist: β1A (ubiquitous) and β1D (cardiac/skeletal muscle specific).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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