Thermo Fisher Scientific ACBD3 Polyclonal Antibody
인간 ACBD3 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot에 적합합니다. N-말단 합성 펩타이드를 면역원으로 사용했으며, PBS와 2% sucrose 용액 내 0.09% sodium azide를 포함합니다. -20°C에서 보관하며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.2–1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide directed towards the N-terminal of human ACBD3 (aa 71–120) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS with 2% sucrose |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2605432 |
Product Specific Information
Peptide sequence:
EARRLEQRWG FGLEELYGLA LRFFKEKDGK AFHPTYEEKL KLVALHKQVL
Sequence homology:
Cow: 100%
Guinea Pig: 100%
Horse: 100%
Human: 100%
Mouse: 100%
Rabbit: 100%
Rat: 100%
Zebrafish: 86%
Target Information
GOLPH1, also known as GCP60, was initially identified as a Golgi protein that interacts with the integral membrane protein giantin and is thought to be involved in maintaining Golgi structure. GOLPH1 also interacts with other Golgi proteins such as Golgin-160, which can be cleaved by caspases-2, -3, and -7, leading to nuclear localization of Golgin-160. The interaction between GOLPH1 and the cleaved Golgin-160 fragments is stronger than with the intact form, regulated by the oxidation state of Cys-463 within GOLPH1. This suggests that nuclear localization of caspase-cleaved Golgin-160 fragments is a coordinated event. Additionally, GOLPH1 interacts with Numb, a cytosolic signaling protein involved in asymmetric cell division of neural progenitor cells, implying that Golgi fragmentation and reconstitution during the cell cycle may regulate Numb signaling and link cell fate with cell cycle progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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