Thermo Fisher Scientific Cdc25A Monoclonal Antibody (DCS-120)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/mg protein lysate

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

DCS-120

Immunogen

Purified recombinant CDC25A

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10980530

Product Specific Information

MA5-13794 targets CDC25A in IP, IHC (P) and WB applications and shows reactivity with Human and Rat samples.

The MA5-13794 immunogen is purified recombinant CDC25A.

Target Information

CDC25a is a member of the CDC25 family of phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. CDC25a is required for progression through G1 and S phases of the cell cycle. CDC25a activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.