Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202, Tyr204) Recombinant Rabbit Monoclonal Antibody (ERK12T202Y204-A11), FITC
FITC로 표지된 재조합 토끼 단일클론 항체로, 인산화된 ERK1/2 (Thr202/Tyr204)를 검출. 사람 및 마우스 시료에 반응. 높은 특이성과 일관성 제공. 유세포분석(Flow Cytometry)에 적합. 4°C 암조건 보관.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 5 µL / 1×10^6 cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ERK12T202Y204-A11 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Thr202/Tyr204 of human phospho Erk1/2 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896832 |
Additional Formats
- Unconjugated (MA5-36895)
- APC (MA5-36898)
- PE (MA5-36896)
- Custom conjugation available upon request
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates.
Key advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
ERK1 and ERK2 are widely expressed kinases involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. These kinases are activated by various stimuli including growth factors, cytokines, and viral infections.
Upon activation, ERKs phosphorylate cytoplasmic targets or translocate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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