Thermo Fisher Scientific AFP Monoclonal Antibody (OTI3F1), TrueMAB
Human AFP 단백질(Alpha Fetoprotein)을 표적으로 하는 마우스 단클론 항체로 Western blot에 최적화됨. TrueMAB 포맷으로 고순도 친화 크로마토그래피 정제. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능. 연구용으로만 사용.
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Applications
- Western Blot (WB)
Tested Dilution: 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3F1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 19–397 of human AFP produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
- For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract, and liver during fetal development.
AFP expression in adults is often associated with hepatoma or teratoma, though hereditary persistence can occur without pathology.
AFP is considered the fetal counterpart of serum albumin; both genes are tandemly located on chromosome 4.
The protein exists in monomeric, dimeric, and trimeric forms, and can bind copper, nickel, fatty acids, and bilirubin.
AFP levels in amniotic fluid are used to screen for spina bifida and anencephaly, while elevated serum AFP is linked to hepatocellular carcinoma (HCC), teratoblastoma, colorectal, pancreatic, and germ cell cancers.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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