Thermo Fisher Scientific CD14 Monoclonal Antibody (Sa14-2)
마우스 CD14 단백질을 인식하는 Rat IgG2a 형 단클론 항체로, Flow Cytometry, Functional Assay, Immunofluorescence에 적합합니다. 단백질 G로 정제된 액상 제품이며, 0.1 mg/mL 농도로 PBS + 0.1% BSA 용액에 보존됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific CD14 Monoclonal Antibody (Sa14-2)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:50 |
| Functional Assay (Functional) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Sa14-2 |
| Immunogen | Murine monocyte marker CD14 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 0.1% BSA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2074183 |
Product Specific Information
- MA1-40064 detects CD14 from mouse samples.
- Successfully used in flow cytometry, functional assays, and immunofluorescence applications.
- Immunogen: mouse monocyte marker CD14.
- Endotoxin level: <24 EU/mg.
- Positive control: RAW cells.
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein expressed on mature monocytes, macrophages, and neutrophils. It functions as a multifunctional lipopolysaccharide receptor and is released into serum as both secreted and cleaved forms. CD14 binds lipopolysaccharide (LPS) via LBP, an acute-phase serum protein. Soluble sCD14 can recognize subtle structural differences in LPS and plays a role in neutralizing serum lipopolysaccharides. Additionally, CD14 binds apoptotic cells and is involved in allergic, inflammatory, and infectious processes. Alternative splicing produces multiple transcript variants encoding the same isoform.
Diseases associated with CD14 dysfunction include Mycobacterium chelonae infection and Croup.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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