Thermo Fisher Scientific SATB1 Monoclonal Antibody (OTI11H4)
인간 SATB1 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC(P), ICC/IF에 사용 가능. 액상 형태로 0.6 mg/mL 농도이며 Affinity Chromatography로 정제됨. PBS/BSA/glycerol buffer에 보존되며, -20°C에서 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI11H4 |
| Immunogen | Full-length protein expressed in 293T cell transfected with human SATB1 expression vector |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2725295 |
Target Information
SATB1 (DNA-binding protein SATB1) is a crucial silencing factor that contributes to the initiation of X inactivation mediated by Xist RNA during embryogenesis and in lymphoma. It binds to DNA at AT-rich sequences and nuclear matrix-associated regions, recognizing the sugar-phosphate structure of double-stranded DNA. SATB1 acts as a transcriptional repressor controlling nuclear and viral gene expression depending on phosphorylation and acetylation status. It plays a role in T-cell maturation and chromatin-loop remodeling. SATB1-binding sites are preferentially targeted by HIV-1 integration machinery, and HIV-1 Tat may interfere with SATB1-mediated repression of IL1 and IL2RA in T-cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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