Thermo Fisher Scientific MCAM Monoclonal Antibody (OTI2G10), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

CD146 (MCAM) is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed at cell-cell junctions in endothelial cells and other normal and malignant cell types.
CD146 functions as a calcium-independent cell adhesion molecule and may act as a signal transduction molecule by recruiting Fyn kinase, leading to phosphorylation of intracellular proteins involved in actin cytoskeleton assembly.
It plays a role in endothelial cell adhesion and vascular integrity, and may facilitate melanoma cell interactions with the vascular system, promoting metastasis.
Additionally, CD146 is active in neural crest cells during embryonic development and acts as a surface receptor triggering tyrosine phosphorylation of FYN and PTK2, with transient increases in intracellular calcium concentration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.