Thermo Fisher Scientific CPI-17 Polyclonal Antibody
CPI-17 단백질에 특이적인 Rabbit Polyclonal 항체로, Western blot 및 ELISA에 적합합니다. Human, Mouse, Rat 시료에서 반응하며, 액상 형태로 제공됩니다. Affinity Chromatography로 정제되어 높은 특이성과 재현성을 제공합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
ELISA
- Tested Dilution: 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–147 of human PPP1R14A (NP_1502811) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.78 mg/mL |
| Purification | Affinity Chromatography |
| Storage buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2804346 |
Product Specific Information
- Immunogen sequence:
MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC GKPVEDFIQE LLAKLQGLHR QPGLRQPSPS HDGSLSPLQD RARTAHP - Positive Samples: MCF-7, Mouse spleen, Rat lung
- Cellular Location: Cytoplasm
Target Information
CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphatase. It was originally identified as a PKC-potentiated inhibitory protein of protein phosphatase-1, dominantly expressed in smooth muscle. Phosphorylation at Threonine 38 by PKC or Rho-kinase enhances inhibitory potency toward myosin phosphatase. CPI-17 may also be involved in calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. It is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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