Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (MEM-147)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Flow Cytometry (Flow)

1-5 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

MEM-147

Immunogen

PHA-activated peripheral blood lymphocytes. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HLA-ABC,uniProtId:P04439-1,ncbiNodeId:9606,antigenRange:1-365,antigenLength:365,antigenImageFileName:MA1-19151_HLA-ABC_P04439-1_House_mouse.svg,antigenImageFileNamePDP:MA1-19151_HLA-ABC_P04439-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:HLA-ABC,uniProtId:P01889-1,ncbiNodeId:9606,antigenRange:1-362,antigenLength:362,antigenImageFileName:MA1-19151_HLA-ABC_P01889-1_House_mouse.svg,antigenImageFileNamePDP:MA1-19151_HLA-ABC_P01889-1_House_mouse_PDP.jpeg,sortOrder:2},{targetFamily:HLA-ABC,uniProtId:P10321-1,ncbiNodeId:9606,antigenRange:1-366,antigenLength:366,antigenImageFileName:MA1-19151_HLA-ABC_P10321-1_House_mouse.svg,antigenImageFileNamePDP:MA1-19151_HLA-ABC_P10321-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1076697

Product Specific Information

This antibody recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types.

The antibody is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.

Target Information

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.