ELK Biotechnology MYPT1 (phospho Thr853) rabbit pAb

제품명

MYPT1 (phospho Thr853) rabbit pAb

대체명 (Alternative Names)

PPP1R12A; MBS; MYPT1; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit

응용 (Applications)

IF; WB; IHC; ELISA

권장 희석 배수 (Recommended Dilutions)

• IF: 1:50–200
• Western Blot: 1:500–1:2000
• Immunohistochemistry: 1:100–1:300
• ELISA: 1:5000
  ※ 기타 응용에서는 아직 테스트되지 않음

면역원 (Immunogen)

The antiserum was produced against synthesized peptide derived from human MYPT1 around the phosphorylation site of Thr853.
AA range: 621–670

보관 조건 (Storage)

-20°C / 1 year

항체 정보

유전자 정보

세포 내 위치 (Cellular Localization)

Cytoplasm; cytoskeleton; stress fiber; along actomyosin filaments

배경 정보 (Background)

Myosin phosphatase target subunit 1 (MYPT1), also known as the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase.
Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho.
The small GTPase Rho is implicated in myosin light chain (MLC) phosphorylation, resulting in smooth muscle contraction and actin–myosin interaction in nonmuscle cells.
Active GTP-RhoA interacts specifically with the myosin-binding subunit (MBS) of myosin phosphatase, regulating MLC phosphorylation.
Rho-associated kinase (Rho-kinase), activated by GTP-RhoA, phosphorylates MBS and inactivates myosin phosphatase.
Overexpression of RhoA or activated RhoA in NIH 3T3 cells increases phosphorylation of MBS.

반응 종 (Species Reactivity)

Human; Mouse; Rat

제품 이미지

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