ELK Biotechnology Survivin (phospho Thr34) rabbit pAb

제품명

Survivin (phospho Thr34) rabbit pAb

대체 명칭

BIRC5; API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin

적용 분야

• WB
• ELISA
• IHC

권장 희석 배율

• WB: 1:500–2000
• IHC-p: 1:50–300
• ELISA: 1:2000–20000

면역원

The antiserum was produced against synthesized peptide derived from human Survivin around the phosphorylation site of Thr34.
AA range: 11–60

보관 및 안정성

• 종: Rabbit
• 보관 조건: −20°C / 1 year

항체 특성

세포 내 위치

Cytoplasm, Nucleus, Chromosome, Centromere, Cytoplasm (cytoskeleton, spindle), Chromosome (centromere, kinetochore), Midbody.
Localizes at the centromeres from prophase to metaphase, at the spindle midzone during anaphase, and at the midbody during telophase and cytokinesis.
Accumulates in the nucleus upon treatment with leptomycin B (LMB), an XPO1/CRM1 nuclear export inhibitor (By similarity).
Localizes on chromosome arms and inner centromeres from prophase through metaphase.
Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase, and at telophase, localizes exclusively to the midbody (PubMed:11084331).
Colocalizes with AURKB at mitotic chromosomes (PubMed:14610074).
Acetylation at Lys-129 directs its localization to the nucleus by enhancing nuclear retention.

배경 정보

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death.
IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain.
The encoded proteins also lack a C-terminus RING finger domain.
Gene expression is high during fetal development and in most tumors, yet low in adult tissues.
Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
[provided by RefSeq, Jun 2011]