Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), Brilliant Ultra Violet 615, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.25 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780

Product Specific Information

The J105 monoclonal antibody reacts with human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. It is expressed predominantly on activated T and B lymphocytes. PD-L1 (B7-H1) and PD-L2 (B7-DC) are its ligands, involved in inhibitory immune signaling.

Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (Product #11-9969).

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested by flow cytometry of normal human peripheral blood cells. Use at 5 µL (0.25 µg) per test.
• Laser Compatibility: Ultraviolet (355 nm) laser required for Brilliant Ultra Violet™ 615 detection.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize non-specific interactions.

• Light Sensitivity: Protect from light.
• Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
• Excitation: 350 nm
• Emission: 615 nm
• Laser: Ultraviolet

Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen™.

Target Information

T lymphocytes initiate cell-mediated immune responses when stimulated by peptides bound to MHC molecules on antigen-presenting cells (APC). PD-1 (PDCD-1) mediates inhibitory signals by binding PD-L1 or PD-L2, limiting immune activation. PD1-deficient mice exhibit hyperactivated immune responses and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates higher in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures.

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