Thermo Fisher Scientific VIM Monoclonal Antibody (OTI5D7), TrueMAB
Vimentin 단백질을 인식하는 Mouse monoclonal antibody. Western blot, IHC, ICC/IF에 사용 가능. 동결건조 형태로 제공되며, 재구성 시 1 mg/mL 농도로 사용. 세포골격 연구 및 단백질 수송 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5D7 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 210–466 of human VIM produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular protein transport between the nucleus and plasma membrane. Unlike other IFPs, vimentin is co-expressed with desmin during early cell development and later replaced by tissue-specific IFPs.
Vimentin plays a role in steroid synthesis regulation by serving as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase leads to filament breakdown and cytoskeletal rearrangement, facilitating cholesterol transport to mitochondria and enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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