Thermo Fisher Scientific CD59 Monoclonal Antibody (MEM-43)
Human CD59 단백질을 인식하는 Mouse IgG2a 단클론 항체로, WB, IHC, Flow Cytometry 등 다양한 응용 가능. 비결합형 액상 형태이며, 단백질 A로 정제됨. CD59 관련 연구 및 세포 표면 단백질 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 1 publication |
| Flow Cytometry (Flow) | 1:5–1:10 | View 5 publications |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 8 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rhesus monkey |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-43 |
| Immunogen | Thymocytes and T lymphocytes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_929142 |
Additional Formats
Product Specific Information
In Western blot, this antibody recognizes CD59 under non-reducing conditions.
A suggested positive control for immunohistochemical applications is tonsil tissue.
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Target Information
CD59 (Protectin) is a small (18–20 kDa) GPI-anchored, ubiquitously expressed inhibitor of the membrane attack complex (MAC). It protects autologous cells from complement-mediated lysis by binding complement C8 and/or C9, preventing pore formation.
CD59 also interacts with the C5b-8 complex, participates in T cell costimulation, and is involved in lymphocyte signal transduction.
Mutations in CD59 can cause CD59 deficiency, leading to hemolytic anemia, thrombosis, and cerebral infarction. Multiple alternatively spliced transcript variants have been identified.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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