Thermo Fisher Scientific ER Polyclonal Antibody
에스트로겐 수용체(ER)를 검출하기 위한 Rabbit Polyclonal 항체로, WB 및 IHC(P) 실험에 적합합니다. Human, Mouse, Rat 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며, 안정적인 신호 검출을 지원합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide surrounding the epitope -LASTN- without phosphorylation of human estrogen receptor |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Human breast carcinoma
- Cellular location: Nucleus, Cytoplasm, Cell membrane
Target Information
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor that, when bound to estrogen hormone, induces a conformational change allowing dimerization and binding to estrogen response elements (ERE) in DNA. ER can regulate gene transcription through coactivator or corepressor recruitment.
There are two forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2). Due to alternative RNA splicing, at least four estrogen receptor-alpha isoforms exist:
- Isoform 1 (66 kDa)
- Isoform 2 (53 kDa)
- Isoform 3 (47 kDa)
- Isoform 4 (35 kDa)
Estrogen receptors are widely expressed in various tissues, essential for sexual development and reproduction, and also function in bone and other tissues. They are involved in pathological processes such as breast cancer, endometrial cancer, and osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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