Thermo Fisher Scientific CENPO Monoclonal Antibody (OTI7F9), TrueMAB
CENPO 단백질을 인식하는 Mouse Monoclonal Antibody로, Western Blot에 최적화되어 있습니다. Human 시료에 반응하며, 동결건조 형태로 제공됩니다. 친화 크로마토그래피로 정제되었으며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7F9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–230 of human CENPO produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Accurate chromosome segregation during mitosis requires proper kinetochore assembly at the centromere of each chromatid, forming a dynamic interface with spindle microtubules. The kinetochore assembly includes the multisubunit CENP-H/I complex, divided into three functional classes. The CENP-O class, represented by CENPO, forms a stable complex essential for kinetochore function and recovery from spindle damage.
CENPO is also identified as an autoantigen in a subset of anti-centromere antibody (ACA)-positive scleroderma patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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