Thermo Fisher Scientific IL-12 p35 Monoclonal Antibody (B-T21 (BT21)), eBioscience

Applications

Tested Dilution

Publications

ELISA (ELISA)

1-4 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

B-T21 (BT21)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468448

Product Specific Information

Description: The B-T21 antibody reacts with the p35 subunit of human interleukin-12 (IL-12). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated B cells. For optimal detection of human IL-12 p70 by ELISA, a combination of purified B-T21 antibody for capture and biotinylated C8.6 antibody for detection is recommended.

Applications Reported: The B-T21 antibody has been reported for use as capture antibody in an IL-12 p70 sandwich ELISA.

Applications Tested: The B-T21 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-12 p70 in combination with the biotinylated C8.6 (13-7129) antibody for detection and recombinant human IL-12 p70 (14-8129) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily. Interleukin-12 alpha chain precursor (IL-12A) is a 35-kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities. Reports suggest that IL-12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK/lymphokine-activated Killer cells. IL-12A stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells. It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein. The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A. Along with EBI3, IL-12A forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.