Thermo Fisher Scientific F4/80 Monoclonal Antibody (BM8), PerCP-Cyanine5.5, eBioscience

Thermo Fisher Scientific F4/80 Monoclonal Antibody (BM8), PerCP-Cyanine5.5, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 661
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• eFluor 506
• eFluor 570
• eFluor 660
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor belonging to the EGF-TM7 family of proteins. It contains seven EGF-like domains, seven transmembrane sequences, and an intracellular C-terminal domain homologous to TM7 superfamily members.
F4/80 is expressed by most mature macrophages and serves as a key marker for this population. Other cells such as peritoneal eosinophils, Langerhans cells, and certain dendritic subtypes also express F4/80. Expression begins early in myeloid development and can be upregulated by M-CSF stimulation.
Some macrophage populations, particularly in lymphoid tissues, may lack F4/80 expression.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse resident peritoneal exudate cells (≤0.5 µg/test; 100 µL final volume; 10⁵–10⁸ cells/test)
Recommended Laser: Blue (Excitation 488 nm; Emission 695 nm)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

F4/80 antigen is a 160 kDa surface glycoprotein, member of the EGF-TM7 family, sharing 68% amino acid identity with human EMR1. Expression varies during macrophage maturation and activation.
It is expressed on mature macrophages in various tissues including Kupffer cells, Langerhans cells, microglia, gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma, and spleen red pulp macrophages.
Also found on some dendritic cells but absent in T cell areas of spleen and lymph nodes.
Recent studies suggest F4/80 may play a role in generating efferent CD8⁺ regulatory T cells.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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