Thermo Fisher Scientific GFAP Monoclonal Antibody (GA5), eFluor 570, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats:

• Unconjugated
• Alexa Fluor 488
• eFluor 615
• eFluor 660
• Request custom conjugation

Product Specific Information

This GA5 monoclonal antibody reacts with glial fibrillary acidic protein (GFAP) from human, mouse, rat, chicken, rabbit, and pig. GFAP is a 49-kDa type III intermediate filament protein expressed in neural tissues, distinguishing astrocytes from other glial cells. The predominant alpha-GFAP isoform is expressed in astrocytes and can co-assemble with vimentin and nestin. GFAP assembly depends on phosphorylation of the N-terminal domain. Mutations in the GFAP gene are associated with Alexander disease and overexpression occurs in certain glial-derived tumors.

Applications Reported:

• Immunohistochemical staining (FFPE tissue)
• Microscopy
• Immunocytochemistry

Applications Tested:

• Tested by IHC on FFPE human tissue using low pH antigen retrieval
• Recommended ≤ 1 µg/mL (optimize by titration)

Filter Recommendation:
Use filters capturing 570 nm emission (e.g., Excitation 545/25, 565LP, Emission 605/70).
Standard Alexa Fluor™ 555 or TRITC filters are acceptable.

Excitation / Emission: 555 nm / 570 nm

Target Information

GFAP (Glial fibrillary acidic protein) is a class III intermediate filament protein expressed in astrocytes and certain glial cells of the CNS, peripheral ganglia, and non-myelinating Schwann cells. It is also expressed in neural stem cells and serves as a key astrocyte marker. GFAP is found in brain tumors, liver Kupffer cells, lens epithelium, and salivary tumors. Mutations in GFAP cause Alexander disease. Alternative splicing produces multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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