Thermo Fisher Scientific IRF8 Monoclonal Antibody (V3GYWCH), APC, eBioscience
인간 및 마우스 IRF8 단백질을 검출하는 단클론 항체로, APC 형광 표지되어 유세포분석(Flow Cytometry)에 최적화되었습니다. 0.25 µg/test 농도로 사용 가능하며, 4°C 암소 보관이 권장됩니다. 수지 크로마토그래피로 정제된 고품질 연구용 항체입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 13 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | V3GYWCH |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573317 |
Additional Formats
- Alexa Fluor 488
- eFluor 450
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
Description:
The V3GYWCH monoclonal antibody reacts with human and mouse interferon regulatory factor 8 (IRF8; ICSBP). IRF8 is a 50 kDa transcription factor that plays a critical role in dendritic cell (DC) development, particularly in plasmacytoid DC (pDC) and CD8a+ DC differentiation. IRF8-/- mice lack both pDC and CD8a+ DC populations, while CD8a- DCs remain but show impaired maturation and function. Retroviral IRF8 transduction restores pDC development from DC progenitors in IRF8-/- mice.
Applications Reported:
Reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested:
Tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00) and protocol.
Refer to BestProtocols®: Protocol B for intracellular (nuclear) proteins.
Recommended use ≤ 0.25 µg per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.
Spectral Information:
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IRF8 (Interferon Regulatory Factor 8)
IRF8 is a transcriptional activator/repressor that binds to upstream regulatory regions of type I IFN and IFN-inducible MHC class I genes. It negatively regulates immune system cells and is involved in CD8+ dendritic cell differentiation via complex formation with BATF-JUNB heterodimer. This complex recognizes the AICE sequence, promoting cooperative binding and gene activation. Mutations in IRF8 are associated with immunodeficiency types 32A and 32B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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