Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Violet 480, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (1.0 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 661 / 737
• Brilliant Violet 421 / 711 / 786
• FITC
• NovaFluor Red 685 / 700 / 755
• NovaFluor Yellow 700
• PE, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600 / 645 / 780
• Request custom conjugation available

Product Specific Information

The 3D12 monoclonal antibody reacts with human CCR7 (CD197), a G-protein-coupled chemokine receptor for CCL19 and CCL21.
CCR7 is expressed on T cells and dendritic cells (DCs) and plays a critical role in migration and maturation processes.
During DC maturation, CCR7 expression increases, influencing chemotaxis to lymph nodes, endocytosis rate, survival, and cellular architecture.
For optimal staining, incubate for at least 45 minutes at 2–8°C.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells.
Use 5 µL (1.0 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test (empirically determined).

Fluorochrome Information:
Brilliant Violet™ 480 (BV480) emits at 479 nm, suitable for cytometers with a 405 nm violet laser.
Not compatible with methanol-based fixation.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Excitation: 440 nm
Emission: 479 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CCR7 is a G protein-coupled receptor induced by Epstein-Barr virus (EBV).
It is expressed in blood, bone marrow, lymph node, and intestine, particularly in lymphoid tissues and activated B and T lymphocytes.
CCR7 regulates memory T cell migration to inflamed tissues and dendritic cell maturation.
Its specific ligands include CCL19 (ECL) and CCL21.
CCR family members (CCR1–5, Duffy antigen) are important for T cell chemotaxis and phagocyte migration to inflammation sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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