Thermo Fisher Scientific DLAT Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 87-270 of human DLAT (NP_001922.2). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DLAT,uniProtId:P10515-1,ncbiNodeId:9606,antigenRange:87-270,antigenLength:647,antigenImageFileName:PA5-120279_DLAT_P10515-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120279_DLAT_P10515-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.07 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2913851

Product Specific Information

Positive test controls include: HeLa, MCF7, HepG2, K-562, Mouse brain, Rat brain. The target is usually found in the following locations: Mitochondrion matrix.

Immunogen sequence: SLPPHQKVPL PSLSPTMQAG TIARWEKKEG DKINEGDLIA EVETDKATVG FESLEECYMA KILVAEGTRD VPIGAIICIT VGKPEDIEAF KNYTLDSSAA PTPQAAPAPT PAATASPPTP SAQAPGSSYP PHMQVLLPAL SPTMTMGTVQ RWEKKVGEKL SEGDLLAEIE TDKATIGFEV QEEG

Target Information

DLAT encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC eventually leads to cirrhosis and liver failure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.