Thermo Fisher Scientific RAP80 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 449-719 of human UIMC1 (NP_057374.3). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RAP80,uniProtId:Q96RL1-1,ncbiNodeId:9606,antigenRange:449-719,antigenLength:719,antigenImageFileName:PA5-120277_RAP80_Q96RL1-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120277_RAP80_Q96RL1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.52 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2913849

Product Specific Information

Positive test controls include: DU145, 293T, HeLa, Mouse thymus. The target is usually found in the following locations: Nucleus.

Immunogen sequence: QLSSSETFDL EREVSPGSRD ILDGVRIIMA DKEVGNKEDA EKEVAISTFS SSNQVSCPLC DQCFPPTKIE RHAMYCNGLM EEDTVLTRRQ KEAKTKSDSG TAAQTSLDID KNEKCYLCKS LVPFREYQCH VDSCLQLAKA DQGDGPEGSG RACSTVEGKW QQRLKNPKEK GHSEGRLLSF LEQSEHKTSD ADIKSSETGA FRVPSPGMEE AGCSREMQSS FTRRDLNESP VKSFVSISEA TDCLVDFKKQ VTVQPGSRTR TKAGRGRRRK F

Target Information

RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.