Thermo Fisher Scientific Smoothelin Polyclonal Antibody
Smoothelin 단백질을 검출하기 위한 Thermo Fisher Scientific의 다클론 항체. Western blot과 IHC(Paraffin)에서 사용 가능. Rabbit IgG 기반 액상 항체로, 고순도 친화 크로마토그래피 정제. 완전 분화된 평활근세포의 표지에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SMTN (Accession P53814), corresponding to amino acid residues S825–P875 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2900568 |
Product Specific Information
Antibody detects endogenous levels of total Smoothelin.
Target Information
Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation.
Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses reveal a broad species distribution of this protein.
Cells with SMC-like characteristics, such as myofibroblasts, myoepithelial cells, and skeletal and cardiac muscle, do not contain smoothelin.
Confocal microscopy and transfection studies show a filamentous organization of smoothelin distinct from desmin and vimentin, and smoothelin colocalizes with actin stress fibers.
Two tissue-specific isoforms have been identified: a 59 kDa isoform specific for visceral SMC and a 100 kDa isoform specific for vascular SMC.
Human smoothelin is encoded by a single copy gene located on chromosome 22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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