Thermo Fisher Scientific TRMT12 Monoclonal Antibody (OTI3C10), TrueMAB
인간 TRMT12 단백질을 인식하는 Mouse IgG2a 단클론 항체로, WB 및 IHC(P)에서 검증됨. Lyophilized 형태로 제공되며, PBS 버퍼(8% trehalose) 포함. TRMT12는 tRNA wybutosine 생합성에 관여하며, 유방암 관련 연구에 활용 가능.
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Thermo Fisher Scientific TRMT12 Monoclonal Antibody (OTI3C10), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3C10 |
| Immunogen | Full-length human recombinant TRMT12 protein produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, an additional desalting step is recommended (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
TYW2 (also known as TRMT12) is an enzyme involved in the wybutosine-tRNA (Phe) biosynthesis pathway.
Wybutosine (yW) is a hypermodified guanosine located adjacent to the anticodon of phenylalanine tRNA, stabilizing codon-anticodon interactions during ribosomal decoding.
TYW2 participates in a multistep enzymatic reaction that ensures accurate translation by stabilizing base-pairing during decoding.
TYW2 amplification has been observed in multiple breast cancer cell lines, and overexpression (>2-fold) was detected in most breast tumor samples, suggesting a potential role in breast cancer progression.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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