
Thermo Fisher Scientific Wilms Tumor 1 (WT1) Recombinant Rabbit Monoclonal Antibody (WT1, 3477R)
Recombinant Rabbit Monoclonal Antibody targeting WT1 protein. Validated for IHC (Paraffin) applications. High specificity and sensitivity with no preservative. Supplied in liquid form, 1 mg/mL concentration, suitable for research use only.
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- 7490-RBM7-P1ABX
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | WT1, 3477R |
| Immunogen | Synthetic peptide corresponding to WT1 residues (within aa1–100) of WT1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C or −80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
The Wilms Tumor 1 (WT1) gene encodes a WPP domain-interacting protein essential for RanGAP nuclear envelope association in root tip cells.
Ran GTPase is involved in nucleocytoplasmic transport, spindle formation, and nuclear envelope reassembly.
WT1 and WIT2 are CC-TMD proteins required for RanGAP nuclear envelope association, distinct from the WIP family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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