Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), eFluor 450, eBioscience
CD45.1 단백질을 인식하는 마우스 단클론 항체(A20 클론)로, eFluor 450 형광체가 결합되어 있습니다. 마우스 백혈구 표지에 적합하며, Flow Cytometry에 최적화되어 있습니다. 405/445 nm에서 여기/방출되며, 4°C 암소 보관이 권장됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 61 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A20 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1272225 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 563
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 650
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 506
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 610-30S
- NovaFluor Blue 660-40S
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
Product Specific Information
The A20 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.1-expressing mouse strains such as SJL/J, DA, STS/A, and RIII. CD45.1 is expressed by all leukocytes in these strains.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤ 0.5 µg per test in 100 µL final volume (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.
- Fluorophore: eFluor® 450 (alternative to Pacific Blue®)
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, constituting ~10% of surface proteins in lymphocytes. It regulates Src-family kinases, plays a role in lymphocyte development and antigen signaling, and exists as multiple isoforms due to alternative splicing. These isoforms vary in extracellular domains and membrane localization, influencing cell-type-specific expression. CD45 also modulates integrin-mediated signaling and apoptosis-related DNA fragmentation. CD45RA is one isoform with restricted expression among lymphoid subtypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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