Thermo Fisher Scientific CD172a (SIRP alpha) Monoclonal Antibody (DH59B)

Applications

Immunohistochemistry (Frozen) (IHC (F))

• Assay-dependent

Flow Cytometry (Flow)

• Neat: 1:10

Product Specifications

Product Specific Information

This antibody recognizes bovine CD172a, also known as SIRP alpha or MyD-1 antigen.
CD172a is a transmembrane signal regulatory protein expressed primarily by macrophages, monocytes, dendritic cells, granulocytes, myeloid progenitors, hematopoietic stem cells, and neurons.

Target Information

SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells, and hematopoietic stem cells.
SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src, and insulin receptor, and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways.
The ligand binding of SIRP alpha to integrin-associated protein CD47 results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of SIRP alpha, which mediates the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2.
Ligation of SIRP alpha with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.
SIRP alpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones, and cell adhesion, and plays a general role in different physiological and pathological processes.
Cancer cells highly express CD47, which activates SIRP alpha and inhibits macrophage-mediated destruction of cancerous cell growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.