Thermo Fisher Scientific TaqMan Salmonella Enteritidis Detection Kit

Applied Biosystems™ TaqMan™ Salmonella Enteritidis Detection Kit

The TaqMan™ Salmonella Enteritidis Detection Kit is part of a comprehensive workflow that enables users to rapidly detect Salmonella Enteritidis in poultry egg and environmental samples. The workflow utilizes TaqMan™ chemistries and the polymerase chain reaction (PCR) to detect Salmonella Enteritidis from egg and environmental samples in approximately 27 hours.
This is the fastest-time-to-results workflow available, shown to be equivalent to the FDA method and approved by the National Poultry Improvement Plan (NPIP) for use in environmental sample detection.

주요 특징

• 결과를 수일이 아닌 수시간 내에 획득하여 계란 출하 속도 향상
• FDA 방법과 동등한 결과 신뢰성 확보
• 높은 특이성으로 비규제 미생물과의 교차 반응 최소화

Test Eggs and Environmental Samples Faster

With as little as 27 total hours to results, egg producers can test implicated eggs in the shortest time currently available—roughly ten times faster than the traditional FDA BAM method.
This allows producers to reduce inventory, lower costs, and deliver the freshest product to market.
For environmental samples, the 27-hour time to results enables rapid identification and corrective actions compared to the traditional three-day method.

Equivalence with FDA Method and NPIP Approved

The FDA has determined that the workflow using the TaqMan™ Salmonella Enteritidis Detection Kit is equivalent in accuracy, precision, and detection sensitivity to the method described in Chapter 5 (Salmonella) of the FDA’s Bacteriological Analytical Manual (BAM, December 2007 Edition).
The NPIP has granted interim approval for this workflow as a rapid screening method for Salmonella Enteritidis in environmental drag swab samples.

Sensitive and Specific Detection

The egg testing method enables accurate detection down to 1–5 cfu of Salmonella Enteritidis from a 1L pooled egg enrichment.
High specificity of molecular PCR technology helps minimize false positives and cross-reactivity with non-regulated organisms.

Automated, Reliable, High-Throughput PCR Sample Preparation

The workflow utilizes MagMAX™ Express-96 Deep Well automated processing equipment and PrepSEQ™ Nucleic Acid Extraction Kit chemistries for fully automated DNA sample preparation.
Up to 96 samples can be processed automatically for PCR detection using the Applied Biosystems™ 7500 Fast instrument.

Simple, Three-Step Procedure

Minimal training or prior experience is needed. The entire procedure consists of three simple steps:

1. Enrich for possible Salmonella Enteritidis — enrich pooled eggs or environmental samples in growth media (24 hrs)
2. Extract DNA — automated extraction using MagMAX™ Express 96 Deep Well Instrument with PrepSEQ™ Nucleic Acid Extraction Kit (1 hr)
3. Run PCR Test — analyze DNA using the TaqMan™ Salmonella Enteritidis Detection Kit and 7500 Fast real-time PCR machine (≈2 hrs)

Qualitative real-time PCR assay for the detection of Salmonella Enteritidis from pre-enriched poultry egg and environmental samples.

For Research Use Only. Not for use in diagnostic procedures.

사양 (Specifications)