Invitrogen™

Ambion™ In Vivo Factor VII Positive Control siRNA

Ambion™ In Vivo siRNA ControlsValidated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcriptsPositive Control siRNAs functionally tested자세히 알아보기

Ambion™ In Vivo siRNA Controls

• Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts
• Positive Control siRNAs functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer™ Select modifications for enhanced specificity or Ambion™ In Vivo modifications for increased stability
• For use in mouse cells

What are Ambion™ In Vivo siRNAs?

Ambion™ In Vivo siRNAs are designed using the proven Silencer_™ Select algorithm and incorporate chemical modifications for superior serum stability with in vivo delivery. The added serum stability does not compromise the performance of the siRNAs. Ambion™ _In Vivo siRNAs have been shown to be non-toxic in vivo (mouse) and non-immunogenic (cell-based assays). In cell-based assays, Ambion™ In Vivo siRNAs exhibit equivalent or better potency than _Silencer_™ Select siRNAs. The result is better confidence in your in vivo RNAi experiments, better knockdown when paired with an efficient delivery solution, and the assurance of limited non-specific effects.

Factor VII (FVII) Positive Control siRNA

Ambion™ In Vivo Factor VII siRNA, In Vivo Ready, provides a positive control for experiments involving Ambion™ In Vivo siRNA delivery to mice. Factor VII (FVII/F7; also known as proconvertin) is a vitamin K-dependent serine protease that functions as a central protein in the coagulation cascade. FVII is synthesized in the liver and secreted to the plasma where it circulates in an inactive form. After delivery of FVII-targeting siRNA to the liver, knockdown can be measured by quantifying FVII protein levels in blood by robust, commercially available chromogenic assays. Moreover, blood samples can be collected from the same animal at multiple time points post-siRNA injection. These features enable much more rapid and efficient knockdown measurements for FVII than for gene targets that require quantitation of mRNA levels in liver tissue, making FVII an ideal control for in vivo siRNA delivery.

Quality Control

Ambion™ synthesizes and purifies each Ambion™ In Vivo siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.

In Vivo Ready Controls

Our In Vivo Ready Control siRNAs are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50 μM in presence of deionized water, in vivo Ready siRNAs contain less than 5.0 mM Na+, less than 0.06 mM K+, and less than 0.02 mM Mg2+.

사양

제어 유형Positive Control

RNAi TypesiRNA

용도(애플리케이션)RNAi, Transfection

라벨 또는 염료Unconjugated

제품라인Ambion™

제품 유형siRNA

순도HPLC

수량250 nmol

배송 조건Room Temperature

TargetFactor VII

Unit SizeEach