Thermo Fisher Scientific HGF Polyclonal Antibody
Human HGF에 특이적인 Rabbit Polyclonal Antibody로, IHC, ICC/IF, ELISA 등 다양한 응용에 사용 가능. Recombinant Human HGF 단백질을 면역원으로 제작. 보존제가 없으며, 장기 보관 시 -20°C에서 보관 권장. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human HGF/Hepatocyte Growth Factor Protein (Met1–Ser728) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787972 |
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human HGF/Hepatocyte Growth Factor.
Target Information
Hepatocyte growth factor (HGF) regulates cell growth, motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor.
It is secreted by mesenchymal cells and acts as a multifunctional cytokine on epithelial-origin cells.
HGF stimulates mitogenesis, cell motility, and matrix invasion, playing a central role in angiogenesis, tumorogenesis, and tissue regeneration.
It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and a 34-kDa beta-chain, forming an active heterodimeric molecule via a disulfide bond.
The protein belongs to the plasminogen subfamily of S1 peptidases but lacks detectable protease activity.
Alternative splicing produces multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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