Thermo Fisher Scientific Bcl-2 Monoclonal Antibody (Bcl-2/100), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

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Immunohistochemistry (IHC)

Assay-Dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

-

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Flow Cytometry (Flow)

Assay-Dependent

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ELISA (ELISA)

Assay-Dependent

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

Bcl-2/100

Immunogen

Rec. Human Bcl-2

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• FITC
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2-7.4

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10597451

Product Specific Information

Description: Bcl-2 is a key regulator of apoptosis and the founding member of Bcl-2 family of apoptosis regulatory protiens. The anti-apoptotic functions of Bcl-2 is achieved by activation or inactivation of an inner mitochondrial permeability transition pore, which is involved in the regulation of matrix Ca2+, pH, and voltage.

Applications Tested: ELISA, Flow Cytometry, Immunohistochemistry, Western Blotting.

Target Information

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.