Thermo Fisher Scientific MCM2 Polyclonal Antibody
Western blot, IHC, ICC, Flow cytometry에 사용 가능한 다종 반응 MCM2 폴리클로날 항체. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 인간, 생쥐, 랫트 시료에 반응. DNA 복제 및 세포주기 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the C-terminus of mouse MCM2 (884–904aa: DLFKFNKFSRDLKRKLILQQF) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5 mg BSA |
| Contains | 0.05 mg sodium azide, 0.05 mg thimerosal |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746760 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
MCM2 (Mini Chromosome Maintenance Protein 2) is involved in regulating DNA replication. Also known as CDCL1, mitotin, and BM28, it is a human nuclear protein crucial for the cell cycle, particularly at the onset of DNA replication and cell division. MCM2 is mapped to chromosome 3q21 and has been identified as a candidate oncogene affected by chromosomal breaks in acute myeloid leukemia (AML).
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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