Thermo Fisher Scientific MCM3 Polyclonal Antibody
MCM3 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF, Flow Cytometry에 사용 가능. 고순도 항원 친화 크로마토그래피 정제 제품으로, 인간·마우스·랫트 반응성. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human MCM3 (13–27aa REAQRDYLDFLDDEE) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5 mg BSA |
| Contains | 0.05 mg sodium azide, 0.05 mg thimerosal |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746761 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) involved in the initiation of eukaryotic genome replication. The hexameric complex formed by MCM proteins (MCM2–7) is a key component of the pre-replication complex (pre_RC) and may participate in replication fork formation and recruitment of other DNA replication-related proteins.
MCM3 interacts directly with MCM5/CDC46 and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. Acetylation inhibits DNA replication initiation and cell cycle progression.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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