Thermo Fisher Scientific Alpha A Crystallin Monoclonal Antibody (1H3.B8), PerCP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Mouse, Rat, Bovine, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1H3.B8

Immunogen

Native Alpha Crystallin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Alpha A Crystallin,uniProtId:P02489-1,ncbiNodeId:9606,antigenRange:1-173,antigenLength:173,antigenImageFileName:MA5-45174_Alpha_A_Crystallin_P02489-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45174_Alpha_A_Crystallin_P02489-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PerCP PerCP PerCP

View additional formats

• Unconjugated
• APC
• FITC
• PE
• Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931628

Product Specific Information

0.5 µg/mL was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Detects approximately 20kDa. Does not cross-react with alpha B-crystallin, beta L-crystallin, beta H- crystallin, gamma -crystallin, HSP25, HSP27 or HSP47 proteins.

Target Information

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander`s disease where it accumulates in brain cells of those afflicted.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.