Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), PerCP-eFluor 710, eBioscience
Mouse CD163 인식 단일클론 항체로, PerCP-eFluor 710 형광체 결합. Flow cytometry 및 IHC에 사용 가능. 고감도 검출 및 안정적 형광 특성 제공. 4°C 보관, 빛 차단 필요.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), PerCP-eFluor 710, eBioscience
Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), PerCP-eFluor 710, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 13 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TNKUPJ |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2716955 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- APC
- Brilliant UV 615 / 661 / 737 / 805
- Brilliant Violet 421
- eFluor 450
- FITC
- PE / PE-Cyanine7 / PE-eFluor 610
- Super Bright 436 / 600 / 702
- Request custom conjugation
Product Specific Information
This TNKUPJ monoclonal antibody recognizes mouse CD163, a 130 kDa surface receptor expressed by specific tissue macrophages such as splenic red pulp macrophages, Kupffer cells, intestinal lamina propria macrophages, and a subset of peritoneal macrophages. Mouse monocytes do not express CD163, and it is not a reliable marker for M2 macrophages.
This antibody detects CD163 on fixed and permeabilized cells, enabling intracellular staining. Aggressive tissue dissociation may reduce surface CD163, in which case intracellular detection is recommended.
Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes; ≤ 0.25 µg/test (100 µL final volume)
Fluorochrome Details:
PerCP-eFluor™ 710 emits at 710 nm and is excited at 488 nm (blue laser). Recommended filter: 710/50 bandpass; alternative: 695/40 bandpass.
Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples may be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333).
Excitation: 488 nm
Emission: 710 nm
Laser: Blue
Target Information
CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein and member of the scavenger receptor cysteine-rich superfamily. It acts as a receptor for hemoglobin-haptoglobin complexes, protecting tissues from oxidative damage and aiding in iron recycling.
CD163 is expressed on human monocytes and macrophages, upregulated by IL-10, IL-6, and dexamethasone, and shed upon stimulation by LPS or PMA. It binds hemoglobin/haptoglobin complexes in a calcium- and pH-dependent manner, initiating intracellular signaling leading to calcium mobilization and cytokine secretion (IL-6, CSF1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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