Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• APC
• Brilliant UV 615 / 661 / 737 / 805
• Brilliant Violet 421
• eFluor 450
• FITC
• PE / PE-Cyanine7 / PE-eFluor 610
• Super Bright 436 / 600 / 702
• Request custom conjugation

Product Specific Information

This TNKUPJ monoclonal antibody recognizes mouse CD163, a 130 kDa surface receptor expressed by specific tissue macrophages such as splenic red pulp macrophages, Kupffer cells, intestinal lamina propria macrophages, and a subset of peritoneal macrophages. Mouse monocytes do not express CD163, and it is not a reliable marker for M2 macrophages.

This antibody detects CD163 on fixed and permeabilized cells, enabling intracellular staining. Aggressive tissue dissociation may reduce surface CD163, in which case intracellular detection is recommended.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes; ≤ 0.25 µg/test (100 µL final volume)

Fluorochrome Details:
PerCP-eFluor™ 710 emits at 710 nm and is excited at 488 nm (blue laser). Recommended filter: 710/50 bandpass; alternative: 695/40 bandpass.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples may be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333).

Excitation: 488 nm
Emission: 710 nm
Laser: Blue

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein and member of the scavenger receptor cysteine-rich superfamily. It acts as a receptor for hemoglobin-haptoglobin complexes, protecting tissues from oxidative damage and aiding in iron recycling.

CD163 is expressed on human monocytes and macrophages, upregulated by IL-10, IL-6, and dexamethasone, and shed upon stimulation by LPS or PMA. It binds hemoglobin/haptoglobin complexes in a calcium- and pH-dependent manner, initiating intracellular signaling leading to calcium mobilization and cytokine secretion (IL-6, CSF1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지