Thermo Fisher Scientific CD105 (Endoglin) Monoclonal Antibody (MJ7/18), APC, eBioscience
마우스 CD105(Endoglin)을 인식하는 MJ7/18 단클론 항체로, APC로 표지되어 있습니다. 주로 유세포분석(Flow Cytometry)에 사용되며, 0.125 µg/test로 검증되었습니다. 혈관내피세포 표지 및 혈관신생 연구에 적합합니다. 4°C 어둠 속 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 26 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MJ7/18 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573154 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488
- Biotin
- eFluor 450
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The MJ7/18 monoclonal antibody reacts with mouse CD105 (Endoglin), a 90 kDa disulfide-linked homodimer expressed by vascular endothelial cells. CD105 is involved in adhesion and embryonic angiogenesis.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on bEnd.3 cells at ≤ 0.125 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.
Fluorescence Properties:
- Excitation: 633–647 nm
- Emission: 660 nm
- Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD105 (Endoglin) is a Type I transmembrane protein highly expressed on vascular endothelial cells. It forms an O- and N-glycosylated homodimer. Upregulation of endoglin has been observed in tumor vasculature and proliferating cells, indicating its role as a proliferation-associated endothelial marker. CD105 binds TGF-β1 and TGF-β3 with high affinity but not TGF-β2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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