Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (HIS.H8), Alexa Fluor 555
6x-His 태그 단백질 검출용 Alexa Fluor 555 결합 단일클론 항체. Western blot, ICC/IF, IHC 등 다양한 응용에 적합. 높은 특이성과 감도로 재조합 단백질 정제 및 검출에 활용. 연구용 전용 제품.
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Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (HIS.H8), Alexa Fluor 555
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:25–1:100 | View publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIS.H8 |
| Immunogen | 6x His synthetic peptide |
| Conjugate | Alexa Fluor™ 555 |
| Excitation / Emission Max | 553 / 568 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2610646 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 647
- Biotin
- DyLight 488
- DyLight 550
- DyLight 650
- DyLight 680
- HRP
- Request custom conjugation
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). This tag is commonly expressed at either the N- or C-terminal regions of recombinant proteins to enable isolation or purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins for which no specific antibody exists by fusing a known epitope to the protein. His-tagged proteins can be purified using Ni²⁺-NTA adsorbents due to the strong and selective chelation between histidine clusters and nickel ions.
His tag-specific antibodies, such as HIS.H8, are used for detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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