Thermo Fisher Scientific CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), Super Bright 436, eBioscience
인간 CD24 항원을 인식하는 eBioSN3 (SN3 A5-2H10) 단클론 항체로, Super Bright 436 형광체가 결합된 유세포분석용 시약입니다. 405 nm 레이저로 여기되어 436 nm에서 방출되며, B세포 발달 및 면역조절 연구에 적합합니다.
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Thermo Fisher Scientific CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), Super Bright 436, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 11 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 436 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioSN3 (SN3 A5-2H10) |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802402 |
Additional Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 805
- eFluor 450
- FITC
- NovaFluor Blue 510 / 555 / 585 / 690 / 760
- NovaFluor Red 725
- NovaFluor Yellow 570 / 590 / 660 / 755
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600 / 645
- Request custom conjugation available
Product Specific Information
The SN3 monoclonal antibody reacts with human CD24 (Heat Stable Antigen, HSA), a 35–50 kDa GPI-anchored molecule expressed on erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage cells. P-selectin (CD62P) is known to bind CD24.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells. Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Super Bright 436 is excited by a 405 nm violet laser and emits at 436 nm. Use a 450/50 bandpass filter or equivalent. Ensure instrument compatibility.
For panels using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD24 is a GPI-anchored glycoprotein expressed during various stages of B-cell development, from pro-B cells in bone marrow to mature surface Ig-positive B cells. Plasma cells show low or no expression. CD24 is commonly found in B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas. It may regulate B-cell proliferation, maturation, and autoimmunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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