Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Super Bright 436, eBioscience
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카탈로그 번호 | CAS 번호 | 설명 | 상태 | 재고 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
62-0251-82 | - | Thermo Fisher Scientific 62-0251-82 CD25 Monoclonal Antibody (PC61.5), Super Bright 436, eBioscience 100 ug pk | 재고문의 | pk | 659,000원 | - | 724,900원 | ||
62-0251-80 | - | Thermo Fisher Scientific 62-0251-80 CD25 Monoclonal Antibody (PC61.5), Super Bright 436, eBioscience 25 ug pk | 재고문의 | pk | 0원 | - | 0원 |
다른 상품 둘러보기
Applications
Tested Dilution
Publications
Immunohistochemistry (IHC)
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View 1 publication 1 publication
Flow Cytometry (Flow)
0.125 µg/test
View 46 publications 46 publications
Neutralization (Neu)
-
View 1 publication 1 publication
Product Specifications
Published species
Mouse
Host/Isotype
Rat / IgG1, lambda
Recommended Isotype Control
Rat IgG1 kappa Isotype Control (eBRG1), Super Bright™ 436, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
PC61.5
Conjugate
Super Bright™ 436 Super Bright™ 436 Super Bright™ 436
View additional formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 563
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 650
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- eFluor 506
- FITC
- Functional Grade
- NovaFluor Blue 585
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 760
- Pacific Orange
- PE
- PE-Cyanine5
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Excitation/Emission Max
413/431 nm View spectra
Form
Liquid
Concentration
0.2 mg/mL
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_2734936
Product Specific Information
Description: The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.
Applications Reported: This PC61.5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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