Thermo Fisher Scientific ARC Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Suggested positive control: Mouse skeletal muscle tissue lysate
• PA5-20305 can be used with blocking peptide PEP-0399

Target Information

ARC (also known as NOL3 - Nucleolar protein 3) has two isoforms:

1. Isoform 1 – Involved in RNA splicing.
2. Isoform 2 – Functions as an apoptosis repressor that blocks multiple modes of cell death.

ARC inhibits extrinsic apoptotic pathways by:

• Interacting with FAS and FADD upon FAS activation, blocking death-inducing signaling complex (DISC) assembly.
• Interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner, limiting soluble CASP8 available for DISC-mediated activation.

ARC also inhibits intrinsic apoptotic pathways through interactions with BAX, resulting in BAX inactivation and prevention of mitochondrial dysfunction and release of pro-apoptotic factors.
Mutations in this gene can result in myoclonus, familial cortical disorder.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.