Thermo Fisher Scientific IL-17F Monoclonal Antibody (eBio18F10), Alexa Fluor 488, eBioscience
마우스 IL-17F 단백질을 인식하는 Alexa Fluor 488 결합 단클론 항체로, 세포내 염색 및 유세포분석에 적합합니다. IL-17F 관련 염증성 질환 연구에 활용되며, 0.5 µg/test 권장. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio18F10 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation/Emission Max | 499/520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_823147 |
Additional Available Formats
Product Specific Information
The eBio18F10 antibody reacts with mouse IL-17F, a 37 kD homodimeric cytokine belonging to the IL-17 family and a signature Th17 marker. IL-17F and IL-17A share strong sequence homology and are co-regulated during Th17 differentiation. Both are expressed in activated human CD4+ T cells and are associated with autoimmune and inflammatory diseases such as rheumatoid arthritis, multiple sclerosis, psoriasis, inflammatory bowel disease, and asthma.
IL-17F induces expression of IL-6, IL-8, GRO-alpha, ENA-78, TGF-beta, MCP-1, G-CSF, GM-CSF, and ICAM-1 in airway epithelial and endothelial cells. It forms both homodimers and IL-17A/F heterodimers, which vary in biological potency.
Applications Reported: Useful for intracellular staining and flow cytometric analysis.
Applications Tested: Tested on in vitro-differentiated mouse Th17 cells and EL-4 cell line. Recommended ≤ 0.5 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize titration for best performance.
Excitation/Emission: 488 nm / 519 nm (Blue Laser).
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-17F (Interleukin 17F, CTLA-8) is a cytokine produced by activated T cells, mast cells, and basophils. It induces cytokine and chemokine expression in epithelial, endothelial, and fibroblast cells, contributing to allergic and inflammatory responses. IL-17F can form homodimers or heterodimers with IL-17A, acting via the IL-17R receptor to promote monocyte and neutrophil recruitment.
IL-17F is implicated in chronic inflammatory diseases such as rheumatoid arthritis, psoriasis, and multiple sclerosis. It is regulated by TGF-beta and IL-23 during Th17 differentiation and inhibits angiogenesis while inducing IL-2, TGFB, and MCP-1 production in endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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