Thermo Fisher Scientific Blood Group Antigen H Type 2 (CD173) Monoclonal Antibody (A51-B/A6)

Applications

Tested Dilution

Publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

2-4 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgA

Class

Monoclonal

Type

Antibody

Clone

A51-B/A6

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

The H antigen is a precursor to the ABO blood groupantigens, present in people of all common blood types. The Bombay phenotype (hh) does not express antigen H on red blood cells, and therefore this type will also lack A or B antigens, similar to the O blood group. However, unlike O group, the H antigen is absent, hence the individuals produce isoantibodies to antigen H as well as to both A and B antigens. If they receive blood from someone with O blood group, the anti-H antibodies will bind to the H antigen on the red blood cells of the donor blood and destroy the RBCs bycomplement-mediated lysis. Therefore, people with Bombay phenotype can receive blood only from otherhhdonors, although they can donate as though they were type O. Some individuals with the blood group A1 may also be able to produce anti-H antibodies due to the complete conversion of all the H antigen to A1 antigen. Production of the H antigen, or its deficiency in the Bombay phenotype, is controlled at the H locus on chromosome 19. The H locus contains threeexonsthat span more than 5kbof genomic DNA, and encodes thefucosyltransferasethat produces the H antigen on RBCs. The H antigen is a carbohydrate sequence withcarbohydrateslinked mainly to protein (with a minor fraction attached toceramidemoiety).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.