Thermo Fisher Scientific Blood Group Antigen H Type 2 (CD173) Monoclonal Antibody (A51-B/A6)

Applications

Immunohistochemistry (PFA fixed) (IHC (PFA))

• Tested Dilution: 1–2 µg/mL

Immunocytochemistry (ICC/IF)

• Tested Dilution: 2–4 µg/mL

Product Specifications

Target Information

The H antigen is a precursor to the ABO blood group antigens, present in people of all common blood types.
The Bombay phenotype (hh) does not express antigen H on red blood cells and therefore lacks A or B antigens, similar to the O blood group. However, unlike O group, the H antigen is absent, leading to production of isoantibodies to antigen H as well as to A and B antigens.
If they receive blood from someone with O blood group, the anti-H antibodies will bind to the H antigen on donor RBCs and destroy them via complement-mediated lysis.
Thus, individuals with Bombay phenotype can receive blood only from other hh donors, though they can donate as if they were type O.
Some individuals with blood group A1 may also produce anti-H antibodies due to complete conversion of all H antigen to A1 antigen.
Production of the H antigen, or its deficiency in the Bombay phenotype, is controlled at the H locus on chromosome 19.
The H locus contains three exons spanning more than 5 kb of genomic DNA and encodes the fucosyltransferase that produces the H antigen on RBCs.
The H antigen is a carbohydrate sequence linked mainly to protein, with a minor fraction attached to a ceramide moiety.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.