Thermo Fisher Scientific VASP Monoclonal Antibody (OTI1H8), TrueMAB
VASP 단백질을 인식하는 Mouse IgG1 단클론 항체로 Western blot, ICC/IF, Flow cytometry에 적합. HEK293T 세포에서 생산된 full-length 인간 VASP 단백질을 면역원으로 사용. 동결건조 형태, PBS buffer 및 trehalose 포함, 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Flow Cytometry (Flow)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H8 |
| Immunogen | Full length human recombinant protein of human VASP produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Vasodilator-stimulated phosphoprotein (VASP) belongs to the Ena-VASP protein family.
Members of this family contain:
- EHV1 N-terminal domain: binds proteins with E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions
- Proline-rich domain: binds SH3 and WW domain-containing proteins
- C-terminal EVH2 domain: mediates tetramerization and binds G and F actin
VASP is involved in filamentous actin formation, cell adhesion, motility, and intracellular signaling pathways regulating integrin–extracellular matrix interactions.
It is regulated by cyclic nucleotide-dependent kinases PKA and PKG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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